The fifth mark was spotted with a reference solution of all four drugs. Students will be expected to receive an 80% or higher on the final lab report. Purpose: In this lab you will separate the components of different pain medications. The eluent used for this experiment was a. Have your lab textbook available for quick reference to specific pages, indicated in red.
The components of the unknown product were identified by comparing characteristic traits, such as Rf, fluorescence and iodine staining, to those exhibited by the standards. The students were able to extract, more or less, six 6 colors and was further broken down into three 3 colors specifically yellow, red and orange. In this experiment, the solid used is silica gel SiO2. I do not remember even seeing a streak above our second spot on the gel plate for the unknown, so this means we may not have spotted enough of the solution on the plate. The plates were then removed from the jar and observed. Then About 20-30 mg of each of the four known compounds will be added to the appropriate test tube, and 20-30 mg of the unknown will be added to the test tube marked 3-unk.
Thin-Layer Chromatography of Indicator Dyes. At the end of the experiment, the students were able to discover the different components of the siling labuyo, its purity and the Rf values of each components of the red siling labuyo. In this method, the components within the mixture are distributed between two phases: a stationary phase and a mobile phase which moves through the stationary. Prepare a Table of Physical Constants for the following compounds. The ingredients of each food coloring were analyzed using the Rf values of all the spots. For each sample, record the data in your notebook, and enter it in your lab report.
Great care was taken to ensure that the plate went in evenly so that the solvent could rise evenly up the plate. If a compound contains ketene, allayed, ester, amide, or ether functional groups, it should be polar. Also, through this experiment, students know some basic indexes to assess the efficiency of pumps used. All standards were spotted twice and the reference samples as well as unknown were spotted six times. The purpose of this experiment is to identify an unknown proprietary drug using thin-layer chromatography. In my lab report for the Density Lab I recorded the steps to the lab. Acetaminophen was found to be the second most polar analgesic drug tested.
Color additives that are exempt from certification include pigments that are derived from natural sources such as vegetables minerals or animals, and man-made counterparts of natural derivatives. The solid may be almost any material that does not dissolve in liquid phase. The movement of the dark purple spots samples during the running of the plate can be observed in the movie. The mobile phase is an organic solvent. The material to be separated is place onto the stationary phase and is then carried along by the mobile phase. Silica gel is a form of silicon dioxide , the silicon atoms are joined via oxygen atoms in a giant covalent structure. The spots are to be evenly spaced, 0.
The last mark was spotted with a reference solution of all four chemicals. Free Essay: The plates were set aside to dry and then visualized under short-wave lamp to locate and circle the spots. It was hard to tell if there some of the streaks actually had spots in them or not, so we may have missed on what should have been a third spot above the acetaminophen spot on our gel plate. Methemoglobin is not a problem in adults since they have an enzyme that converts methemoglobin back to hemoglobin. Discussion Using the Thin Layer Chromatography experiment, caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Results Sample Distance between start line and spot Distance between start line and front line Rf Aspirin 3.
Chromatography lab report - Find out main recommendations how to. This related to Tylenol in the reference plate with an Rf value 0. Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two different phases between which are to be partitioned. According to research done after the experiment, the testing successfully determined which compounds the sample drugs contained. This is in general terms, not specifics for this lab. They are shown in Table 2. They were then placed in a jar with iodine where some spots developed a brownish color.
Results Sample Distance between start line and spot Distance between start line and front line Rf Aspirin 3. Thin Layer and Column Chromatography. The components of the mixture are absorbed by the stationary phase to different degrees and it is thus the various rates of migration for each component on the adsorption the slower the material passes through the system. The surface of the silica gel is very polar , this is because of the hydroxyl igroup it can formed hydrogen bonds with suitable compounds around it as well as van der waals dispersion forces and dipole-dipole attraction. An analytical report is generated for each project. Procedure Chromatograph method is a method of separating mixtures of two or more compounds.
Chromatographic are used extensively in organic chemistry laboratories for routines analysis. This is the stationary phase. Therefore when energy leaves one place it has to have another place to go to, it maintains a constant rate. The second plate was spotted with Anacin, Bufferin, Excedrin, Tylenol, and reference. Now let us report the experimental results gathered in this study.