Properly record all observations Sketches and diagrams should be neatly drawn in the same colour as seen through the microscope, keeping the relative size of the bacteria and tissue cells. If you need a short break, wash or sanitize your hands and leave the room. What does this nitrate test mean? Which organism--left or right--is + for coagulase? Important steps in the use of a microscope: There are usually three objectives lenses in a microscope. Students will sit in their serial order according to the number and shall work in small group allotted to one teacher. The Gram positive bacteria have a thick layer of peptidoglycan, which is able to hold the crystal violet-iodine complex within the cell wall when alcohol is applied.
Put all used pipettes, swabs and microscope slides in the biohazard disposal containers located in the front of the room and under the hood. Which of the following not only reveals cell morphology but will provide additional information about the specimen: a. Also, if high magnification is to be used, more light is needed. Student must possess lead pencil, eraser and coloured pencils red, blue, violet, green, brown, yellow and a piece of thin clothe for cleaning and wiping the microscopic slides and cover slips etc. Which of the following functions of light allows the greatest magnification by a microscope? Culture tubes should be stored upright in plastic beakers, while Petri plates should be stacked and incubated upsidedown lid on the bottom.
Definition Ordinary hand washing does not remove all bacteria from the skin neither does a surgical scrub. Since some of the microorganisms used in this class are pathogenic or potentially pathogenic opportunistic , it is essential to always follow proper aseptic technique in handling and transferring all organisms. The slide is then suspended over a water bath with some sort of porus paper over it, so that the slide is steamed. Which of the following serves as the main energy source for most living cells? You conclude that the cells are most likely: a. It is also dried and fixed by flaming. When you come into the lab, always pull out and organize any culture tubes or Petri plates you have in the incubator from previous labs.
Check up the microscope, clean the eyepiece and the objectives with cloth and see that the condenser diaphragm and mirror are correctly adjusted. What is the purpose of the condenser on a light microscope? Safety of cafeteria eating utensils Definition Chapter 7 experiment 2: Term General Concepts 7 General Concepts from Selected Laboratory Exercises How were the following features of bacteria demonstrated in the laboratory? Definition Bacillus anthracis Term Exercise 8. Helps control contrast on the specimen Term Exercise 1. Which reaction is + for catalase? What is it used for? Place a drop of oil on the smear ensure the stained smear is on the top of the slide. Definition 1 Candida albicans 2 Thrush, vaginitis, cutaneous candidiasis 3 Budding from mother cell Term Exercise 5.
Definition 1,000, 100, 450 Term Exercise 1. The Ixodes tick is a transmission factor for which of the following organisms? A volume of one cubic inch is sufficient to contain approximately nine trillion average-sized bacteria. Never attempt to bring the object into focus by lowering the body tube while looking through the eye piece. Definition 25 degrees Celsius Term Exercise 5. What would you expect to see if you prepare a gram stain slide of the gram negative bacillus, Escherichia coli: a. Minor adjustments in fine focus and light contrast may be needed. The average diameter of prokaryotic cells is: a.
Definition Base - Support for microscope Substage light - Illumination source located on base of microscope which shines light up through the condenser to the slide and object being viewed Stage - platform that supports slides for viewing Mechanical stage and control knobs - Apparatus which holds slides in place and which is used to adjust the X and Y position of the slide on the stage Condenser - Substage lens that focuses the light on the specimen so that each of its points are evenly illuminated Condenser knob - used to adjust the condenser up and down Iris diaphragm lever - Opens and closes the condenser, thus regulating the amount of light shining through the condenser onto the object. Definition Gram Stain - The Gram stain reaction is dependent upon the nature of the cell wall of the bacterium. What do you call these filaments found in fungi? Resistance of bacteria to antibiotics Definition Term General Concepts 4 General Concepts from Selected Laboratory Exercises How were the following features of bacteria demonstrated in the laboratory? What are these holes called? Malachite green is applied to the slide, which can penetrate the tough walls of the endosppores, staining them green. It is also differential media because it allows one to determine the hemolytic patterns Term Exercise 7. Laboratory Rules For the safety and convenience of everyone working in the laboratory, it is important that the following laboratory rules are observed at all times: Place only those materials needed for the day's laboratory exercise on the bench tops. Which of the following signs and symptoms is not linked to Haemophilus influenzae? An average coccus-shaped spherical bacterium see Fig. It is important to know what microorganisms are growing in each tube or on each plate.
Work this dilution problem, solving for the number of bacteria per ml. Definition Protection against phagocytosis inside the body and dehydration outside the body Term Exercise 4. No smoking, eating, drinking, or any other hand to mouth activity while in the lab. Make sure the entire cord is on the bench top and not hanging down where it could be caught by a leg. Definition Tuberculated chlamydospore Term Exercise 5. It should not touch the slide Look through the ocular, bring the smear to sharp focus using the fine adjustment. To adjust them, first find the proper distance between your eyes and the eyepieces by closing one eye and slowly moving your head toward that eyepiece until you see the complete field of view - about 1 inch away.
You are examining a drop of aquarium water under the microscope and observe green cells without nuclei. Definition Streptococcus pneumonia and Klebsiella pneumoniae Term Exercise 4. Remember: One cannot compare the effectiveness of different antibiotics by their zones of inhibition in this test Term General Concepts 1 General Concepts from Selected Laboratory Exercises How were the following features of bacteria demonstrated in the laboratory? Name this fungus used in lab. This worm is a----hookworm, tapeworm, fluke, filarial worm, pinworm? Disinfect the bench tops with isopropyl alcohol before and after each lab. No answer in TestBank 50. What is the total magnification if you were viewing a specimen using this objective lens? Definition Bacilli rod , cocci spherical , spiral helical Term Exercise 1. Which one of the following allows bacterial cell motility? Late comers are not allowed to enter the class.
Definition The term bacteriostatic is used for inhibition of bacterial growth microbiostatic would be used for any microbe, fungistatic for fungi, etc. Using a wax marker, properly label all inoculated culture tubes or Petri plates with the name or the initials of the microorganism you are growing, your initials or a group symbol, and any other pertinent information. You must wear shoes that cover the tops of your feet to prevent injury from broken glass, spilled chemicals, and dropped objects. What is the stain procedure that malachite green is used for? Definition Flagella Arrangements: a Monotrichous - Single flagella at one end polar b Lophotrichous - Many from one end polar c Amphitrichous - One or more at each end bi-polar d Peritrichous - Many randomly all over non-polar Term Exercise 4. Looking from the side refer figure over the oil immersion objective till it touches the oil on the smear and is just above the slide. Definition Check the ones with the zones.
The first part of each lab period will be used to complete and record the results of prior experiments. Good streak plate or bad streak technique? If a cell is expending a lot of energy, which of the following would be the best nutrient molecule to be broken down for immediate energy: a. Definition Once focused with a low power objective, the image remains always in focus for the other objective lenses, or very nearly in focus. The microscope in essence, consist of the following : The Support System in the form of the base, body and the stage. Light waves thus travel from the glass slide, into glass-like oil, into the glass lens without being scattered or distorting the image Fig. Do not place regular trash such as Kim wipes and paper towels in the biohazard containers.